FRAP analysis of wild type and mutant lamin As. A and B) Single z-plane confocal images of GFP-tagged (A) wt and (B) N195K lamin A expressing cells. Images were captured before (t = -5) and immediately after (t = 0) photobleaching of an area of the nuclear periphery, and at 5 min intervals thereafter. The bleach region is boxed in red. C) Graphs of mean (± s.e.m) relative fluorescence in the bleach area during FRAP, averaged over 9 cells each. In each graph, data for wt (black) and a mutant (red) lamin A are compared.