FLIP analysis of wild type and mutant lamin As. A) Single z-plane confocal images of a GFP-tagged wt lamin A expressing cell captured before (left) and immediately after (right) a round of photobleaching of an area of the nuclear periphery (red box). Fluorescence was also recorded for an unbleached area (blue box) of the nuclear periphery, and a region of the nucleoplasm (green box). Bar = 10 μm B) Graphs of mean (± s.e.m) relative fluorescence in the bleach area (red) during successive rounds of FLIP, and in unbleached regions of the nuclear periphery (blue). and the nucleoplasm (green). Data are averaged over 10 cells each for wt lamin A and for the L530P and N195K mutant lamin As.