Figure 10

Effect of PE and FN on the expression of smooth muscle differentiation markers and MyoD. Preconfluent, serum-deprived fibroblasts were stimulated with either PE (5 μM) or FN (1 μM) for up to 48 h. Expression of markers was determined by Western blot analysis of whole cell lysates as described in Methods. A, smooth muscle α actin (SMα actin). B, caldesmon. C, smooth muscle myosin heavy chain (SM-MHC). D, MyoD. VSMC lysate is shown as a positive control in panel A, B, C and D. The bar graph represents quantitation of protein bands by densitometric analysis of blots obtained from three different experiments. * Value significantly higher than the corresponding vehicle, p < .05.