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Figure 2 | BMC Cell Biology

Figure 2

From: Activation of α1A-adrenergic receptor promotes differentiation of rat-1 fibroblasts to a smooth muscle-like phenotype

Figure 2

Stimulation of α 1A -AR mediates the effect of PE on DNA and protein synthesis. A, Effect of prazosin (α1-AR antagonist) and propranolol (β-AR antagonist) on PE-induced inhibition of DNA synthesis. Cells were treated with prazosin and/or propranolol for 30 min before the addition of 5 μM PE for 18 h. [3H]thymidine incorporation was measured as described in Methods. Data are expressed as dpm of [3H]thymidine incorporated per well. Values are the mean ± S.E. of three independent experiments performed in quadruplicates on different batches of cells. * Value significantly different from the corresponding value obtained without PE treatment, p < .05. B, Effect of prazosin and propranolol on PE-induced increase in protein synthesis. Experimental conditions were similar as described in A. [3H]leucine incorporation was measured as described in Methods. Data are expressed as dpm of [3H]leucine incorporated per well. Values are the mean ± S.E of three independent experiments performed in quadruplicates on different batches of cells. * Value significantly different from the corresponding value obtained without PE treatment, p < .05.

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