Figure 5

Effect of prazosin and SQ22536 on PE-and FN-induced morphological change in rat-1 cells. Preconfluent, serum-deprived cells were either stimulated with 5 μM PE and/or 1 μM FN for different periods of time (Fig 5B) and/or pretreated for 30 min with 100 nM prazosin (Fig 5A) or 25 μM SQ22536 (Fig 5C), and then further incubated with 5 μM PE and/or 1 μM FN for 24 h. Morphological studies were performed as described in Methods. The same morphological patterns were obtained in three different experiments.