Figure 8
Effect of PE on the expression of cyclin D1, PCNA Cdk 2, and Cdk 4. Preconfluent, serum-deprived cells were stimulated with PE (5 μM) for 6, 12, 24 and 48 h. Cell-cycle regulators expression was determined by Western blot analysis of whole cell lysates as described in Methods. The bar graph represents quantitation of protein bands by densitometric analysis of blots obtained from three different experiments. * Value significantly different from the corresponding vehicle, p < .05.