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Figure 9 | BMC Cell Biology

Figure 9

From: Activation of α1A-adrenergic receptor promotes differentiation of rat-1 fibroblasts to a smooth muscle-like phenotype

Figure 9

Time course of the effect of PE on the expression of the smooth muscle differentiation markers and MyoD. Preconfluent, serum-deprived fibroblasts were stimulated with PE (5 μM) for up to 48 h. The protein expression of smooth muscle differentiation markers and MyoD was determined by Western blot analysis of whole cell lysates as described in Methods. A, smooth muscle α actin (SMα actin). B, caldesmon. C, smooth muscle myosin heavy chain (SM-MHC). D, MyoD, E, vimentin. VSMC lysate is shown as a positive control. Percentages representing the quantitation of protein bands by densitometric analysis of blots obtained from three different experiments are indicated on top of each immunoblot.

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