Treatment | G0/G1 phase | S phase | G2/M phase | % inhibition (S) |
|---|
vehicle | 9269 ± 0.5 | 319.5 ± 13.5 | 238.5 ± 23.5 | 0 % |
2 μM PE | 9770 ± 9.0 | 54.0 ± 2.0 | 125 ± 21.0 | 83% |
5 μM PE | 9699 ± 29.0 | 59.0 ± 4.0 | 170 ± 11.0 | 81% |
10 μM PE | 9761.5 ± 89.5 | 47.5 ± 13.5 | 110.5 ± 39.5 | 85% |
- Cells were subjected to flow cytometric DNA analysis as described in Methods. Different concentrations of PE inhibited the re-entry of the cell population into S phase of the cell cycle. Data show the number of cells in each phase of the cell cycle. Values are the mean ± S.E. of three independent experiments performed in duplicate on different batches of cells (Total events per cycle is 10,000 cells).
