Activation of α1A-adrenergic receptor promotes differentiation of rat-1 fibroblasts to a smooth muscle-like phenotype

Table 2 Effect of PE and cAMP elevating agents on viability of rat-1 fibroblasts using the hemocytometer trypan blue method.

Treatment	Total number of cells	Blue cells	% of viability
Vehicle	78	8	90%
5 μM PE	142	10	92%
10 μM PE	87	12	86%
10 μM FN	78	8	90%
20 μM 8-cpt-cAMP	84	4	96%
20 μM 8-Br-cAMP	66	2	97%

Cells were incubated for 18 h with PE (5 and 10 μM), forskolin (FN), 8-cpt-cAMP and 8-Br-cAMP. The percentage of viable cells was calculated. Values are representative of five different experiments.

ISSN: 2661-8850