Figure 1

Sam68 overexpression inhibits cell proliferation independently of RNA binding. A. Tet-off cells conditionally expressing wild-type (WT) or RNA binding-defective (G178E) HA-epitope tagged Sam68 were grown in the presence or absence of 100 ng/ml doxycycline for 24 h. HA-Sam68 expression was assessed by immunoblotting with anti-HA antibody. B. Cells conditionally expressing wt or G178E HA-Sam68 or no cDNA (neo) were plated at low density and cultured in the presence or absence of doxycycline. Adherent cells were collected by trypsinization and counted at the indicated times after plating. Results are representative of three separate experiments.