Figure 2

Sam68 overexpression inhibits G1 to S phase progression and decreases G1 cyclin levels. A. Cells in the presence or absence of doxycycline were synchronized in mitosis by nocodazole treatment and released into G1 phase by drug washout. Cells were lysed in SDS-PAGE sample buffer 8 h after release and lysates were immunoblotted with the indicated antibodies. BrdU incorporation was assessed 24 h after release and quantitated by flow cytometry. B. Cells were released from mitosis and total RNA was prepared at the indicated times. Levels of the indicated transcripts were determined by RT-PCR with specific primers. Results are representative of three separate experiments.