Figure 4

Effect of PBP10 on fMLF and WKYMVM induced mobilization of CR3 to the neutrophil cell surface. Neutrophils were incubated with or without PBP10 (1 μM) at 37°C for 5 minutes after which the cells were challenged with fMLF (100 nM) or WKYMVM (100 nM) for another 10 minutes. After fixation of the cells with paraformaldehyde and labeling with anti-CR3 antibody, the amount of CR3 on the cell surface was determined by FACS analysis. A representative histogram of PBP10 effect on WKYMVM mediated CR3 exposure is shown (inset), and the exposure of CR3 after cell stimulation with fMLF and WKYMVM is expressed as percent of control (without PBP10; mean ± SD of four independent experiments). *p < 0.05 compared to WKYMVM without PBP10.