Figure 7
From: Fas induces apoptosis in human coronary artery endothelial cells in vitro
Stimulation of Caspase 1 and Caspase 3 activities in human coronary artery endothelial cells by activation of Fas. Cultured human coronary artery endothelial cells were incubated for 20 hours in the presence and absence of anti-Fas clone CH-11 activating antibody, and were then detached from the culture surfaces. Over the subsequent hour, the activities of Caspase 1 (CASP1) and Caspase 3 (CASP3) were determined as described in Figure 6. Data are expressed as substrate cleaved per 106 cells, relative to unstimulated control cultures (CTL). Bars are the mean + S.E.M. of at least three determinations; * = p < 0.05 versus unstimulated control (CTL) by ANOVA and Student-Newman-Keul's test.