Figure 3

LF2000 increases IR tyrosine phosphorylation in a dose-dependent fashion. Cho.hIR cells were treated for four hours with the indicated volumes of LF2000 which were previously diluted as described in the methods section. Lysates were blotted for phosphotyrosine and bands corresponding to the phosphorylated IR were quantified by densitometric scanning. Data are from one experiment performed in duplicate which was repeated with similar results three times. *p < 0.05; **p < 0.01 compared to control. Insert: shows a typical western blot of phosphotyrosine on the insulin receptor in cells treated with the indicated volumes of LF2000.