Figure 5

Scrib and LPP are localized in cell-cell contacts but are dispensable for targeting each other to these structures. Upper panels: MDCKII cells, grown on glass coverslips, were double labelled with anti-LPP antibodies (left panel) and anti-Scrib antibodies (right panel). Lower panels: MDCKII stable cell lines, expressing GFP-fusion proteins containing wild-type human LPP (upper left panel), LPP with a mutated carboxy-terminus (T610A) (upper right panel), human wild-type Scrib (lower left panel), or Scrib with a deletion of all its PDZ domains (lower right panel), were grown on glass coverslips (Scrib) or on Transwell-Clear polyester membranes (LPP). GFP-fluorescence was visualized by epifluorescence microscopy (Scrib) or by confocal microscopy (LPP).