Figure 7

Scrib can recruit LPP to an ectopic location in vivo through its PDZ domains. CV-1 cells were transiently co-transfected with Xpress-hScrib-mito or Xpress-hScribdPDZ-mito, and GFP-fusions of wild-type full length human LPP, or LPP with a mutated carboxy-terminus (T610A). Xpress-hScrib-mito and Xpress-hScribdPDZ-mito are composed of the human full length Scrib protein with or without its PDZ domains, respectively, which is fused to an Xpress-epitope-tag at its amino-terminus, and to an ActA-derived mitochondrial membrane anchor at its carboxy-terminus, Cells were stained with an anti-Xpress antibody to detect Xpress-Scrib(dPDZ)-mito. Immunofluorescence and GFP were visualized by epifluorescence microscopy. The focal adhesion localization of the GFP-LPP proteins is not visible in these pictures because a focal plane corresponding to mitochondrial staining is shown.