Figure 2

Co-immunoprecipitation of Abi1 and HSPC300 with Scar Homology Domain. Cos 7 fibroblasts transiently transfected as indicated. Protein G beads were used to immunoprecipitate Myc-Scar1 SHD, Myc-Scar2 SHD or Myc-Scar3 SHD from lysates in the presence (+) or absence (-) of anti-Myc (9E10) monoclonal antibody. Empty pRK5-Myc vector was used as an additional negative control. (A) HA-Abi1 was detected in the beads plus antibody fractions for Myc-Scar1 SHD, Myc-Scar2 SHD, and Myc-Scar3 SHD, but not in the negative controls. (B) HA-HSPC300 was detected in the beads plus antibody fraction of immunoprecipitations from cells co-transfected only with Myc-Scar1 SHD, Myc-Scar2 SHD, and Myc-Scar3 SHD, but not empty vector controls or in the absence of 9E10 antibody.