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Figure 4 | BMC Cell Biology

Figure 4

From: Inclusion of Scar/WAVE3 in a similar complex to Scar/WAVE1 and 2

Figure 4

IRSp53 interacts with Scar/WAVE1, 2 and 3. Equivalent amounts of GST-IRSp53 or constitutively active GST-L61 Rac were used for pull-down assays from lysates of Cos cells transfected with Myc-Scar1, 2 or 3. Bead fractions and whole cell lysates, indicating loading with each Scar/WAVE isoform, were analysed by SDS-PAGE and immunoblotting with monoclonal (9E10) anti-Myc antibody. None of the Scar/WAVE isoforms bound to active Cdc42, but all were detected in GST-IRSp53 bead fractions.

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