Figure 11
From: Ca2+ regulation in the absence of the iplA gene product in Dictyostelium discoideum
Basal and cAMP-induced Mn2+-influx. Influx was assayed by quenching of Fura2-dextran fluorescence. (A, B) The response of wild type amoebae is shown for comparison; 1 μM Mn2+ ± 1 μM cAMP was added. iplA - cells in nominally Ca2+-free buffer were challenged with 100 μM Mn2+ ± 1 μM cAMP at t7 (closed symbols); when 1 μM Mn2+ was added (open symbols) no influx was detected (C, D). After preincubation with EGTA influx was observed at 1–2 μM Mn2+ ± 1 μM cAMP (E, F). Fluorescence intensity at 360 nm excitation is shown as mean ± s.e.m