Figure 6
From: Ca2+ regulation in the absence of the iplA gene product in Dictyostelium discoideum
Chemotaxis of iplA - cells is impaired in the intracellular presence of a Ca2+-buffer. Wild type and mutant amoebae were loaded with Fura2-dextran and their ability to protrude pseudopods towards a cAMP-filled glass capillary was compared to that of untreated cells. In both strains the presence of the chelator in the cytosol led to a decrease in the fraction of cells extending pseudopods and migrating towards the cAMP source.