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Figure 2 | BMC Cell Biology

Figure 2

From: Identification and characterization of Iporin as a novel interaction partner for rab1

Figure 2

2 Genomic structure and functional elements of Iporin. (A) Schematic representation of the human Iporin gene located on chromosome 9. The protein coding exons of Iporin are shown as shaded boxes numbered one to eleven, non-coding sequences are white. The gene (~14 kb) encodes a protein of 1516 amino acids. (B) Domains and structural motifs of Iporin. The most interesting domains within Iporin are the SH3 (aa 1447–1500) and the RUN domain (aa 1031–1175). The proline-rich motifs (pP, aa 429–442; aa 1311–1319) are possible interaction motifs for SH3 or WW domain containing proteins. The glutamic acid-rich region (pE, aa 1236–1252) is negatively charged with possibly stabilizing function. (C) Alignment of RUN domains from related proteins. The sequence corresponding to the RUN domain of Iporin (aa 1031–1175, human) was aligned to the RUN domains present in AI840675 (aa 309–453, mouse), NESCA (aa 53–197, human), RPIP8 (aa 52–189, human), Rabip4 (aa 31–163, mouse) and Rufy1 (aa 31–163, human) (GenBank accession numbers, XP_131380.2, BAA77507.2, NP_006686.1, CAC17732.1 and AAK50771.1, respectively). RUN domains were detected using the Motif Scan software http://www.expasy.org. Hydrophobic residues (V, L, I, F, Y, W, M, C) are show as boxes, conserved positions are shaded. A-F are the six conserved blocks, and distances between these blocks are indicated by the numbers of residues in parentheses. (D) Alignment of sequenced clones identified by phage display using the Iporin SH3 domain. Four different clones were isolated, whereby the number of independent clones, which contained the same sequence, are in brackets. The conserved consensus motif is shaded. Ψ = aliphatic amino acid; X2 = two individual amino acids.

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