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Figure 1 | BMC Cell Biology

Figure 1

From: Trapping of normal EB1 ligands in aggresomes formed by an EB1 deletion mutant

Figure 1

Live imaging of EB1-ΔN2-GFP aggregates in transfected COS-7 cells. Panel A. Single frame from additional file 1 showing the structure of EB1-ΔN2-GFP aggregates. Bar = 5 μm. Panel B. Single frame from additional file 2, sequence 1 showing aggresome (arrows) behaviour in two transfected cells. Panel C. Single frame from additional file 2, sequence 2 that shows the cells from sequence 1 imaged using phase-contrast microscopy to reveal adjacent untransfected cells. This sequence was begun 30s after the completion of sequence 1. Arrows indicate aggresome location in the transfected cells. Bar = 10 μm. Panels D-F. Three frames from additional file 3, sequence 1 showing the extension of a linear structure from an EB1-ΔN2-GFP aggregate. Panels G-I. Three frames from additional file 3, sequence 2 showing the retraction of this structure. Bar for D-I = 5 μm. Times shown are relative to the first frame in each sequence. Panels A and D-I were obtained using a 63X oil immersion lens. Panels B and C were obtained using a 40X dry lens.

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