Figure 7
From: Trapping of normal EB1 ligands in aggresomes formed by an EB1 deletion mutant
Dynein/dynactin subunits are present in EB1-ΔN2-GFP aggresomes. Panels A-C. Cells expressing EB1-ΔN2-GFP were fixed and co-immunostained for GFP (green, panel A) and p150glued (red, panel B). A merged image is shown in panel C. p150glued is seen in aggresomes. Bar = 10 μm. Panels D-F. A single 0.365 μm confocal section through a cell expressing EB1-ΔN2-GFP. p150glued immunoreactivity in aggresomes (panel E) is heterogeneous but only seen in association with GFP immunoreactivity (panels D and F). Centrosomal immunostaining is also observed (arrow). Bar = 5 μm. Panels G-L. Cells expressing EB1-ΔN2-GFP were fixed and co-immunostained for GFP (green, panel G) and either CDIC (red, panel H) or p50dynamitin (red, panel K). Merged images are shown in panels I and K. Heterogeneous CDIC and p50dynamitin immunostaining is seen in aggresomes and at centrosomes. Bars = 10 μm. Images in panels A-C and G-L are projections of confocal image stacks.