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Figure 1 | BMC Cell Biology

Figure 1

From: A strategy to study tyrosinase transgenes in mouse melanocytes

Figure 1

Transfer of YAC Tyr transgenes from a Tyrc/ Tyrcbackground to a Tyrc-32DSD/ Tyrc-32DSDbackground. Southern blot analysis of representative mice obtained during the breeding program, using the RFLP [9] and p19ArfE1 probes. Lanes: (M) 1 kb-ladder (Invitrogen), (1) YAC Tyr YRT2/ [9]; Tyr c-32DSD/ Tyr c, (2) YRT2/ ; Tyr c-32DSD/ Tyr c-32DSD, (3) / ; Tyr c-32DSD/ Tyr c, and (4) / ; Tyr c-32DSD/ Tyr c-32DSD. Note: indicates the absence of the transgene. Tg: YAC Tyr transgene derived signal. Tyr c: endogenous albino tyrosinase locus. p19: The p19Arf E1 probe (kindly provided by M. Malumbres), detecting exon 1 of the single-copy p19arf gene, was employed as an internal control for DNA loading and comparisons.

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