Figure 1From: A strategy to study tyrosinase transgenes in mouse melanocytesTransfer of YAC Tyr transgenes from a Tyrc/ Tyrcbackground to a Tyrc-32DSD/ Tyrc-32DSDbackground. Southern blot analysis of representative mice obtained during the breeding program, using the RFLP [9] and p19ArfE1 probes. Lanes: (M) 1 kb-ladder (Invitrogen), (1) YAC Tyr YRT2/∅ [9]; Tyr c-32DSD/ Tyr c, (2) YRT2/∅ ; Tyr c-32DSD/ Tyr c-32DSD, (3) ∅/∅ ; Tyr c-32DSD/ Tyr c, and (4) ∅/∅ ; Tyr c-32DSD/ Tyr c-32DSD. Note: ∅ indicates the absence of the transgene. Tg: YAC Tyr transgene derived signal. Tyr c: endogenous albino tyrosinase locus. p19: The p19Arf E1 probe (kindly provided by M. Malumbres), detecting exon 1 of the single-copy p19arf gene, was employed as an internal control for DNA loading and comparisons.Back to article page