Figure 2

3D visualization of tracks over time. The 2 dimensional culture surface from within an imaged well is represented in the plane of the paper with the time dimension (labelled as "scan") represented by the third dimension coming up toward the reader. More distant time is perceived to be deeper beneath the surface of the paper using exaggerated perspective. Cell object positions appear at each scan where objects met the combined criteria of having an area less than 3000 sq microns and a track length of at least 3 segments. Position markers are colored according to fence size with blue indicating smaller, and red indicating larger fence size. Stationary objects are readily recognized by persistently straight "vertical" paths toward the reader through time, because their x, y coordinates changed very little. A total elapsed time of approximately 75 hours is represented from the "bottom" to the "top" of the 3D representation. Four different surface coatings are shown as indicated. This display was created using Spotfire visualization software (http://www.Spotfire.com), an important tool for accessing, analysing and reporting on the multi-parametric data from the automated system.