Figure 6
GSNO accelerates the rate of M2/M4 AChR-stimulated [35S]GTPγS binding to rat forebrain membranes but has no effect on basal guanine nucleotide exchange rate. Membranes were preincubated for 30 min in control conditions or in the presence of 0.5 mM GSNO, and time-response for basal and CCh-stimulated (10-4 M] [35S]GTPγS binding was determined, as detailed in the Methods section. Values represent specific binding (mean ± SD of duplicate determinations) from one representative experiment that was replicated three times with similar outcome.