Table 2 Effects of GSNO treatment on agonist (CCh) dose-response parameters in [35S]GTPγS binding assays of hM4 cell line membranes. Membranes were preincubated in control conditions or in the presence of 0.5 mM GSNO for 30 min. Values are mean ± SE from three to four independent experiments performed in duplicate. Emax is expressed in percentage over basal with nonspecific binding subtracted.
| Â | Control | GSNO | ||
|---|---|---|---|---|
CHO cell line | log(EC50) | Emax (%) | log(EC50) | Emax (%) |
hM4-WT-A1 | -4.87 ± 0.14 | 586 ± 31 | -4.94 ± 0.09 | 674 ± 23 |
hM4-WT-E5 | -4.87 ± 0.05 | 213 ± 3 | -4.98 ± 0.13 | 276 ± 10** |
hM4-WT-C2 | -5.21 ± 0.20 | 134 ± 3 | -5.27 ± 0.20 | 154 ± 8* |
hM4-C133S-H2 | -5.13 ± 0.10 | 198 ± 4 | -5.12 ± 0.11 | 235 ± 6** |