Table 1 Summary of average lifetimes for GFP emission under various conditions
From: The use of time-resolved fluorescence imaging in the study of protein kinase C localisation in cells
Transfection | Fig | Treatment | Area analysed | Avg Lifetime (ns) |
|---|---|---|---|---|
GFP-PKC | 2 | None | cytoplasm | 2.2 |
None | nucleus | 2.2 | ||
3 | TPA | cytoplasm | 2.2 | |
TPA | nucleus | 2.3 | ||
GFP-PKC/DsRed-cav | 4 | None | cytoplasm | 2.4 |
None | cytoplasm | 2.5 | ||
5 | Ca2+ | cytoplasm | 1.6* | |
Ca2+ | nucleus | 2.0 | ||
6 | TPA | cytoplasm excluding peripheral | 1.5* | |
TPA | nucleus | 2.0 | ||
TPA | peripheral | 1.8* | ||
7 | Bradykinin | cytoplasm | 1.6* |