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Table 1 Summary of average lifetimes for GFP emission under various conditions

From: The use of time-resolved fluorescence imaging in the study of protein kinase C localisation in cells

Transfection Fig Treatment Area analysed Avg Lifetime (ns)
GFP-PKC 2 None cytoplasm 2.2
   None nucleus 2.2
  3 TPA cytoplasm 2.2
   TPA nucleus 2.3
GFP-PKC/DsRed-cav 4 None cytoplasm 2.4
   None cytoplasm 2.5
  5 Ca2+ cytoplasm 1.6*
   Ca2+ nucleus 2.0
  6 TPA cytoplasm excluding peripheral 1.5*
   TPA nucleus 2.0
   TPA peripheral 1.8*
  7 Bradykinin cytoplasm 1.6*
  1. *reduced lifetime indicating PKC-GFP-DsRed-cav interaction