Figure 2

AIDA-1c expression is highest in neuronal tissue and is present in cancer lines. (A) A Multi-Tissue Northern blot (Clontech) was probed with an AIDA-1c specific probe. Re-probing of the same blot with a β-actin probe (bottom panel) is shown, verifying the presence of RNA in each lane. (B) (Upper panel) Nested PCR using AIDA-1c specific primers was conducted on cDNA obtained from a variety of cancer cell lines: SK-MEL-2 (melanoma), NIH:OVCAR-3 (ovarian), HCT-15 (colon), A-549 (non small cell lung carcinoma), MDA-MB-231 (breast), MCF-7 (breast). The positive control lane used AIDA-1c cDNA as the template. (Lower panel) HeLa expresses AIDA-1c. HeLa RNA was subjected to RT-PCR using an AIDA-1c specific primer. No product is observed in the reaction lacking reverse transcriptase (-RT).