Figure 4

Effects of guanylic nucleotide starvation using mutants of the purine nucleotide biosynthesis pathway. A. Growth of an ade8 Δ gua1 Δ double mutant and an isogenic ade8 Δ single mutant on SD medium containing the indicated purines. The SD + ADE + GUA medium contains 25% Adenine / 75% Guanine ratio (total purine concentration of 0.3 mM). B. The ade8 Δ gua1 Δ strains was grown in SD medium containing 25% Adenine / 75% Guanine ratio to OD_{600 nm} = 0.2; then shifted into the indicated medium. After 4 hours of incubation at 30°C, the percentage of cells with two daughters among budding cells was counted. More than 200 cells were counted for each condition. C. Growth of the WT strain, isogenic single amd1 Δ or aah1 Δ mutant strains and amd1 Δ aah1 Δ double mutant strain on SD medium, SD medium supplemented with adenine or SD medium supplemented with hypoxanthine. D. WT strain (left) and amd1 Δ aah1 Δ double mutant strain (right) were grown in SD medium to OD_{600 nm} = 0.2 and then shifted into the indicated medium. After 4 hours of incubation at 30°C, the percentage of cells with two daughters among budding cells was counted. More than 200 cells were counted for each condition.