Figure 4
From: Localization of plasma membrane t-SNAREs syntaxin 2 and 3 in intracellular compartments
Syntaxin 2 localized in perinuclear membrane vesicles and syntaxin 3 localized in the TGN in NEM treated NRK cells. The NRK cells were incubated in the presence of 1 mM NEM for 15 minutes and then further incubated for two hours. Both syntaxin 2 and 3 accumulated into intracellular compartments in the presence of NEM (A,B). The cells were double stained with syntaxin 2 (A) or syntaxin 3 (B) anti-serum and LRSC-conjugated goat anti-rabbit IgG as well as antibodies against transferrin receptor (Ox26) (C) and TGN38 (D) mouse monoclonal antibodies and FITC-conjugated goat anti-mouse IgG. The yellow colour in merged images (E and F) reveals the co-localization. Confocal fluorescence images were viewed using a Leica SP1 microscope system. Bars,10 μm.