Figure 6
From: Localization of plasma membrane t-SNAREs syntaxin 2 and 3 in intracellular compartments
Syntaxin 2 and 3 staining coincides with actin at cortical regions. The NRK cells were fixed, permeabilized and then double stained with syntaxin 2 (A) or 3 (C) anti-serum, LRSC-conjugated goat anti-rabbit IgG as well as guinea pig anti-α-ADF (B,D) and FITC-conjugated donkey anti-guinea pig IgG. Conventional fluorescence images were viewed using an Olympus AX70 fluorescence microscope. Bars, 10 μm.