Figure 8
From: Localization of plasma membrane t-SNAREs syntaxin 2 and 3 in intracellular compartments
Syntaxin 2 or 3 staining does not accumulate into actin containing aggregates after depolymerization of actin filament. The NRK cells were treated with 10 μM cytochalasin D for 30 minutes to disrupt the actin filaments. The cells were fixed, permeabilized and then stained with syntaxin 2 or 3 anti-serum and LRSC-conjugated goat anti-rabbit IgG as well as Oregon Green phalloidin. Conventional fluorescence images were viewed using an Olympus AX70 fluorescence microscope. Bars, 10 μm.