Sequencing of the mutant prelamin A cDNA and fibroblast morphology in the subject with HGPS. (A) Sequence of cDNA encoding mutant prelamin A in the subject with HGPS. Mutant prelamin A cDNA sequence from nucleotide 1804 to the end of the coding region and the corresponding deduced amino acid sequence are shown. (B) Confocal microscopic analysis of dermal fibroblast in primary culture from a control (a and b) and the subject with HGPS (c and d). Labelling was performed with anti-lamin A/C antibodies. Note the presence of irregularly shaped nuclear envelopes in many of the subject's fibroblasts. (C) Double label immunofluorescence microscopic analysis of fibroblasts from an unaffected control (a to c) and subject with HGPS (d to f) labelled with anti-lamin B1 (red)) and anti-vimentin (green) antibodies. Bars, 10 μm.