Chic impairs nuclear export. Localization of GFP reporter appended with wild-type NLS and NES (B, D, F) or mutant NESP12 (A, C, E) in salivary gland cells. In wild-type (A) and chic mutants (C, E) NLS-NESP12-GFP is predominantly localized to the nucleus. In wild-type salivary glands NLS-NES+-GFP is mostly found in the cytoplasm (B). In contrast, in chic mutants, chic2/chic221 and chic01320 homozygotes, NLS-NES+-GFP is predominantly nuclear (D, F).