Skip to main content
Figure 2 | BMC Cell Biology

Figure 2

From: Regulation of actin cytoskeleton architecture by Eps8 and Abi1

Figure 2

Abi1 modulates the morphology of actin structures induced by Eps8. Actin structures induced by Eps8 and Abi1 in (A-C) B16F1, (D-F) MDA-MB231, and (G-I) MDA-MB231BO cells. Distribution of Eps8-myc (A,D,G,J) revealed by 9e10 anti-c-myc antibody, Abi1-GFP (B,E,H), and Abi1DY-GFP (K) revealed by GFP, and actin (C,F,I,L) revealed by phalloidin staining in fixed cells. (A-I) Simultaneous expression of Eps8 and Abi1 induces the formation of actin cables in B16F1, MDA-MB231, and MDA-MB231BO. Eps8 and Abi1 co-localize in association with actin cables (arrows) but Abi1 is not enriched with Eps8 in filopodia (arrowheads in A-C). (J-L) Formation of actin cables in B16F1 cells is dependent on the interaction of Eps8 and Abi1. Abi1DY does not co-localize with Eps8 and does not induce actin cable formation. Scale bar is equal to 10 μm.

Back to article page