Figure 2

Growth on collagen I impairs HDMEC proliferation as measured by BrdU incorporation. HDMEC were seeded onto plastic or dishes coated with collagen I, starved and then stimulated with 50 ng/ml VEGF either alone or in combination with 10, 50 or 250 ng/ml TSP-1 (panel A), 16, 80 or 400 ng/ml IP-10 (panel B) or 100, 500 or 2500 ng/ml recombinant endostatin (panel C) for 48 h. BrdU labeling mixture was then added and the cells were incubated overnight at which time incorporated BrdU was quantitated using the BrdU Cell Proliferation ELISA (Roche). All bars represent the mean and standard error of triplicate samples. All samples were normalized to VEGF-induced BrdU incorporation and statistical significance (* = p < 0.05) was determined using unpaired t-tests as compared to VEGF.