Figure 5
From: The response of VEGF-stimulated endothelial cells to angiostatic molecules is substrate-dependent
HDMEC treated with TSP-1 and IP-10, but not endostatin show slight decreases in procaspase-3 expression. HDMEC were seeded on either plastic or collagen I coated tissue culture dishes, and following removal of non-adherent cells by washing, were stimulated with either 50 ng/ml VEGF alone or in combination with 10, 50 or 250 ng/ml TSP-1 (panel A), 16, 80 or 400 ng/ml IP-10 (panel B), or 100, 500 or 2500 ng/ml recombinant endostatin (panel C) for 48 h. Cells were collected by trypsinization or collagenase treatment and total protein lysates were generated. Western blots for procaspase-3 were performed as described in materials and methods. Blots were stripped and reprobed for tubulin as a loading control.