Figure 13

Immunoblotting of MPRO extracts with various HP1 antibodies. Samples: H, HeLa; O, MPRO undifferentiated cells; RA, MPRO granulocytes (differentiated 4 days). Panels: a, Coomassie blue stained membrane; b, monoclonal anti-HP1 β (Chemicon); c, monoclonal anti-HP1 γ (Chemicon). Note that MPRO HP1 β appears to be of higher mol wt, than that of HeLa cells. Also note the apparent reduction of the amount of MPRO HP1 γ during RA-induced differentiation.