Figure 9

Immunostaining of human blood buffy coat with various HP1 antibodies. Columns: Cy3, antibody staining viewed by conventional epifluorescent microscopy; DAPI (DNA staining). Note that the monocytic nuclei exhibit staining with all monoclonal anti-HP1 antibodies (Chemicon); whereas, the granulocytic nuclei only reveal staining with anti-HP1 γ. Anti-HP1 α, β and γ appear to stain cytoplasmic granules. Scale bar: 10 μm.