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Figure 7 | BMC Cell Biology

Figure 7

From: Extracellular degradation of lipoprotein lipase in rat adipose tissue

Figure 7

Analysis by SDS-PAGE of the cleavage of 125I-LPL in conditioned medium and the effect of an MMP inhibitor Explants of adipose tissue from fed rats were incubated as in Figure 1 for four hours and the medium was collected. 125I-LPL was then incubated at 4°C (to minimize the risk of conformational changes) in this medium with or without the MMP inhibitor GM6001 (5 μg/ml). Lane 1 – fresh medium, lane 2 – conditioned medium, lane 3 – conditioned medium + inhibitor. The lower band in lane 1 is a proteolytic fragment of LPL that is always present in preparations of the enzyme from bovine milk [23].

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