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Table 1 Changes in LPL mass during incubation. Explants of rat adipose tissue were incubated for three hours as in Figure 1. The tissue explants and the medium were recovered and then the vessel was rinsed out with warm (~80°C) SDS solution. This was then suitably diluted with Triton X-100 to match the composition of the medium used for the ELISA. Mean ± SEM of five parallel incubations.

From: Extracellular degradation of lipoprotein lipase in rat adipose tissue

 

LPL mass (ng/g tissue)

 

Before incubation

After incubation

Adipose tissue

1882 ± 13

665 ± 11*

Culture medium

 

26 ± 6

Washing solution

 

12 ± 3

Total

1882 ± 13

702 ± 8 *

  1. * P < 0.001 compared with before incubation