Figure 2

GFP-MFP partially co-localizes with the endogenous peroxisomal matrix protein catalase. Onion epidermal cells expressing GFP-MFP were fixed and then probed with monoclonal antibodies raised against catalase. GFP-MFP fluorescence (A), mouse anti-catalase antibody staining (B), and an overlay of the two images (C) are shown in a region of a single transformed cell by epifluorescence microscopy. The acquisition of these images was set at low exposure to capture the brightly fluorescing peroxisomes. The less intensely fluorescing MTs in this GFP-MFP expressing cell were not visible at this exposure. Bar, 4 μm.