Figure 1

Schematic representation of the different functional domains of RasGEFM identified with MotifScan. The RasGEFM protein contains the following recognizable modules: 6 proline rich regions, a RasGEF N-terminal domain, a DEP domain and a RasGEF catalytic domain. The sequence of the RasGEFM catalytic domain (aminoacids 673–863) has been aligned, using the ClustaIW program, with representative RasGEFs proteins from different organisms and others putative Dictyostelium RasGEFs. Identical and conserved aminoacid residues are boxed in dark or light gray respectively. Accession numbers are referred to GeneBank: [AAN46882 D.d.GEF M, AAN46874 D.d.GEF E, AAN46879 D.d.GEF J, A41216 D.m.Sos, P28818 R.n.p140, A38985 H.s. CDC25]