Figure 13From: A novel Dictyostelium RasGEF required for chemotaxis and developmentChemotaxis of wild-type and HSB61 cells. Cells were developed in shaken suspension, either in the presence or absence of exogenous cAMP pulses, plated on coverslips and tested for chemotaxis towards a microcapillary diffusing cAMP. Upper and middle panels show AX2 or HSB61 cells starved for 5 hours, bottom panel shows HSB61 cells treated with cAMP pulses for 10 hours (see additional files 1, 2, 3). Higher magnifications of each cell sample are shown on the right. Numbers: time in minutes, starting after positioning of the microcapillary (0' time).Back to article page