Figure 8
From: A novel Dictyostelium RasGEF required for chemotaxis and development
Cyclic AMP stimulation of ( A ) adenylyl and ( B , C ) guanylyl cyclase activity. (Squares) AX2 or (triangles) HSB61 cells were treated with cAMP pulses for (A, B) 5 or (C) 9 hours. In conjunction with a cAMP pulse (arrows), samples were taken at the time indicated in the abscissa for determining total concentration of (A) cAMP or (B, C) cGMP. Cyclic-nucleotides were measured using the radioimmunoassay kit as described in the section Material and Methods. Representative experiments are shown.