Figure 6

3D-FISH with the mouse major satellite DNA probe. For each cell type, maximum intensity xy-projections (top) and 3D-reconstructions (bottom) are shown. In the projections, DNA counterstain (TO-PRO3) is shown in red and FISH signals are false-colored in green. Overlap of both signals in chromocenters leads to the intense yellow color. Scale bars represent 5 μm in the respective projection. The 3D-reconstructions (not to scale) are shown together with xy, xz and yz maximum intensity projections in the background. Chromocenters in the reconstructions are shown as solid green structures, while the nuclear border is presented as a transparent shell. Note the differences in number and size of chromocenters in the various cell types and the differences in nuclear shape.