Figure 8

GFP-CpnA labels endosomes, lysosomes, and phagosomes. Cells expressing GFP-CpnA were incubated with red fluorescent nanobeads for 2 hours, flattened with agarose, fixed, and imaged for both the beads and GFP-CpnA simultaneously using confocal microscopy. The same cells are displayed twice; GFP-CpnA only is shown on the left (A, C, E), while both the red beads and GFP-CpnA are shown to the right (B, D, F). Cells expressing GFP-CpnA were incubated with Alexa Fluor-594-labeled yeast for 1 hour, flattened with agarose, fixed, and imaged for both the yeast and GFP-CpnA simultaneously using confocal microscopy. One cell is displayed twice: GFP-CpnA only (G) and to the right, both GFP-CpnA and yeast (H).