Table 3 Summary of the Sub-cellular Characteristics of Embryos Exposed to Anoxia.
Normoxia | 30 Minutes Anoxia (Brief) | 6–12 Hours Anoxia (Intermediate) | 24–72 Hours Anoxia (Long-term) |
|---|---|---|---|
Prophase Blastomeres: | |||
Chromosomes Throughout Nucleoplasm | Chromosomes Aligned Near Nuclear Membrane | Chromosomes Aligned Near Nuclear Membrane | Chromosomes Aligned Near Nuclear Membrane |
Histone H3 Phosphorylation | Histone H3 Phosphorylation | Histone H3 Dephosphorylation | Histone H3 Dephosphorylation |
Metaphase Blastomeres: | |||
Metaphase Plate Normal | Metaphase Plate Normal | Metaphase Plate LengthReduced | Metaphase Plate LengthReduced |
Histone H3 Phosphorylation | Histone H3 Phosphorylation | Histone H3 Phosphorylation | Histone H3 Dephosphorylation |
Microtubules Polymerization | Detectable Microtubule Depolymerization | Microtubule Spindle Perimeter Reduced | Microtubule Spindle Perimeter Reduced |
SAN-1 Localizationat Kinetochore and Nucleoplasm | SAN-1 Localization at Kinetochore or Lateral Extensions | SAN-1 Localization at Kinetochore or Lateral Extensions | SAN-1 Reduced Localization at Kinetochore |
SAN-1 not essential | SAN-1 essential | SAN-1 essential | SAN-1 essential |
Interphase Blastomeres: | |||
Chromosomes Throughout Nucleoplasm | Chromosome Condensation | Chromosome Condensation and Alignment Near Nuclear Membrane | Chromosome Condensation and Alignment Near Nuclear Membrane |