Figure 7

The experiments outlined in Fig. 6 revealed that CD11b expression in DMSO-treated HL60 cells is a multi-step process involving an intermediate "primed" state. (A,B). The "restimulated" (solid diamonds) and "twice restimulated" (solid circles) cells both showed a similar increased rate of CD11b expression (fraction of CD11b^High cells) relative to the "native" (open triangles) or "untreated" (open squares) groups. (C,D). This accelerated kinetics was sustained for up to four days in DMSO-free medium but was gradually lost beyond four days. Duplicates are presented, where solid circles, open diamonds, open triangles, solid squares, and solid triangles (--) represent cells cultured for 1, 2(for C)/4(for D), 5, 6, and 7 days in control medium before restimulation with 0.8% DMSO, respectively. Open squares indicate cells of the native population that have never been previously treated with DMSO nor FACS sorted. Each data point represents a flow cytometry measurement obtained from 10,000 cells.